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KMID : 0388320240310010051
Journal of Korean Academy of Fundamental Nursing
2024 Volume.31 No. 1 p.51 ~ p.59
Checking the Cleanliness of Surgical Instruments Using the Adenosine Triphosphate-Bioluminescence Assay
Park Hyun-Hee

Leed Sung-Soon
Kim Su-Mi
Abstract
Purpose: Cleaning is crucial for reprocessing reusable surgical instruments (RSIs). In this study, we used theadenosine triphosphate (ATP) bioluminescence method to assess the cleanliness of RSIs according to thecleaning method used.
Methods: From April 3 to June 2, 2023, surgical sets, including hemostatic forceps, needleholders, operating scissors, dressing forceps, and suction tubes, that arrived at the decontamination room in thecentral sterile supply department of one general hospital were selected through convenience sampling. For eachof the three cleaning processes, 30 cleaned RSIs were sampled with test swabs for inspection, and the relativelight unit value was measured using ATP test equipment.

Results: Suction tubes, hemostatic forceps, and needleholders ranked low in cleanliness (x2=11.69, p=.020), and the contamination rate after mechanical cleaning wassignificantly lower than that after manual cleaning (x2=31.34, p<.001).

Conclusion: Surgical instruments treatedby manual processing and those containing tubes were associated with the low cleanliness. Therefore, it isnecessary to check whether surgical instruments, including tubes, are manually washed at an appropriate level,and regular monitoring will be required.
KEYWORD
Adenosine triphosphate, Biofilms, Decontamination, Surgical instruments, Surgical site infection
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